In the potassium hydroxide (KOH) process, a microscope is used to examine samples prepared using the KOH technique. This process is commonly employed in medical mycology to detect fungal elements in clinical samples, such as skin, hair, or nail specimens.
The steps involved in the KOH process are as follows:
Sample Collection: The clinician or medical professional collects the relevant clinical sample from the patient, such as a skin scraping, hair plucked from the affected area, or nail clippings.
Sample Preparation: The collected sample is then mixed or treated with a solution of potassium hydroxide (KOH). KOH is an alkaline solution that acts as a clearing agent, helping to dissolve or digest the non-fungal components of the sample, such as skin cells and debris. The process leaves behind the fungal elements, making them more visible for examination.
Microscopic Examination: After the KOH digestion process, a drop of the prepared sample is placed on a microscope slide and covered with a coverslip. The slide is then placed under a microscope for examination.
Microscopic Observations: The microscopic examination allows the laboratory personnel, typically a medical technologist or a medical mycologist, to visualize the fungal elements present in the sample. They will look for characteristic structures such as hyphae, spores, or other fungal structures to identify the type of fungal infection.
The KOH process is a rapid and straightforward method for the detection of fungal infections. It helps to clarify the sample and make the fungal elements stand out, facilitating their identification under the microscope. The technique is often used in cases where a superficial fungal infection is suspected, like dermatophytosis (ringworm), candidiasis (yeast infection), or other skin, hair, or nail fungal infections.
Using a microscope in the KOH process is essential for accurate and timely diagnosis, allowing appropriate treatment to be prescribed based on the identified fungal species.
We have several options for KOH microscopes in stock. The Olympus CX23 is a good example and a great choice!